Key Total magnification = 400x Objective magnification = 40x P = Perichondrium Cap = Capsule * = Capsule of a lacuna, but with the lacuna and contained chondrocyte not included within the thickness of the section Ch = Chondrocyte L = Lacuna. Introduction to Histology: Cartilage and Bones Observations Take a picture and insert it in the appropriate section below. Also record the total magnification needed to best view the object. Total magnification = (ocular lens power) × (objective lens power) Hyaline cartilage Indicate the chondrocytes, lacunae, and perichondrium. Magnification used:40x Fibrocartilage Indicate the chondrocytes.
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Hyaline Cartilage (40x objective lens) In this high magnification view of hyaline cartilage, you can see the cytoplasm and nuclei of shrunken chondrocytes within round lacunae. The variations in staining of the matrix are due to varying proportions of eosinophilic collagen fibers and basophilic polysaccharides. Stain = H&E.. Hyaline Cartilage: Cross Section (Mammalian) a. Connective Tissue: Hyaline Cartilage 40x b. Connective Tissue: Hyaline Cartilage 100x c. Connective Tissue: Hyaline Cartilage 200x d. Connective Tissue: Hyaline Cartilage 200x e. Connective Tissue: Hyaline Cartilage 200x f. Connective Tissue: Hyaline Cartilage 400x g. Connective Tissue: Hyaline.
